References

1

Aubele, M., Zitzelsberger, H., Szücs, S., Werner, M., Braselmann, H., Hutzler, P., Rodenacker, K., Lehmann, L., Minkus, G., Höfler, H.: Comparative FISH analysis of numerical chromosome 7 abnormalities in 5 $\mu$m and 15 $\mu$m paraffin-embedded tissue sections from prostatic carcinoma. Histochem Cell Biol 107 (1997) 121-126

2

Becker Jr., C.R.L., Mikel, U.V., Oliver, LTC W.R., Sesterhenn, I.A.: Enumeration of interphase chromosomes: Comparison of visual in situ hybridization and confocal fluorescence in situ hybridization. Anal Quant Cytol Histol 18 5 (1996) 405-409

3

Babu, V.R., Miles, B.J., Cerny, J.C., Weiss, L., van Dyke, D.L.: Cytogenetic study of four cancers of prostate. Cancer Genet. Cytogenet. 48 (1990) 83-87

4

Irinopoulou, T., Vassy, J., Beil, M., Nicolopoulou, P., Encaoua, D., Rigaut, J.P.: Three-dimensional DNA image cytometry by confocal scanning laser microscopy in thick tissue blocks of prostatic lesions. Cytometry 27 (1997) 99-105

5

Johnson, G.D., de C Nogueira Araujo, G.M.: A simple method of reducing the fading of immunofluorescence during microscopy. Immunol Methods 43 (1981) 349-350

6

Kriete, A. Ed.: Visualization in biomedical microscopies - 3-d imaging and computer applications. VCH, Weinheim (1992)

7

McNeal, J.E.: Cancer volume and site of origin of adenocarcinoma of the prostate: relationship to local and distant spread. Hum. Pathol. 23 (1992) 258-266

8

Micale, M.A., Mohamed, A., Sakr, W., Powell, I.J., Wolman, S.R.: Cytogenetics of primary adenocarcinoma. Cancer Genet Cytogenet 61 (1992) 165-173

9

Pinkel, D., Straume, T., Gray, J.W.: Cytogenetic analysis using quantitative high-sensitivity fluorescence hybridization. Proc Natl Acad Sci USA 83 (1986) 2934-2938

10

Preston Jr., K. and Siderits, R.: New technologies for 3-d data analysis in histopathology. Anal Quant Cytol Histol. 14 5 (1992) 398-406

11

Scardino, P.T., Weaver, R., Hudson, M.A.: Early detection of prostate cancer. Hum. Pathol. 23 (1992) 211-222

12

Serra, J.: Image analysis and mathematical morphology. Academic Press, London (1982)

13

Sheppard, C.J.R and Gu, M.: 3-d transfer functions in confocal scanning microscopy. In: [6] 251-281

14

Tekola, P., Baak, J.P.A., Beliën, J.A.M., Brugghe, J.: Highly sensitive, specific, and stable new fluorescent DNA stains for confocal laser microscopy and image processing of normal paraffin sections. Cytometry 17 (1994) 191-195

15

Tekola, P., Baak, J.P.A., van Ginkel, H.H.A.M., Beliën, J.A.M., van Diest, P.J., Broeckert, M.A.M., Schuurmans, L.T.: Three-dimensional confocal laser scanning DNA ploidy cytometry in thick histological sections. J. Pathol. 180 (1996) 214-222

16

Vrolijk, H., Sloos, W.C.R., van de Rijke, F.M., Mesker, W.E., Netten, H., Young, I.T., Raap, A.K., Tanke, H.J.: Automation of spot counting in interphase cytogenetics using brightfield microscopy. Cytometry 24 (1996) 158-166

17

Zhu, Q., Tekola, P., Baak, J.P.A., Beliën, J.A.M.: Measurement by confocal laser scanning microscopy of the volume of epidermal nuclei in thick skin sections. Anal. Quant. Cytol. Histol. 16 2 (1994) 145-152

18

Zitzelsberger, H., Szücs, S., Weier, H.-U., Lehmann, L., Braselmann, H., Enders, S., Schilling, A., Breul, J., Höfler, H., Bauchinger, M.: Numerical abnormalities of chromosome 7 in human prostate cancer detected by fluorescence in situ hybridization (FISH) on paraffin-embedded tissue sections with centromere-specific DNA probes. J. Pathol. 172 (1994) 325-335

 

Figure 1:   Graphical user interface for 3-D image display

 

Figure 2:  

a b

Functional tablet for user interaction, a) Segmentation and semi-automatic count and journaling b) Display, visual count and journaling

 

Figure 3:   Graphical user interface for volume display by rendering and slicing

 

Figure 4:   Graphical user interface for volume display by rendering and slicing

 

Figure 5:  Gallery of a selected box

 

Figure 6:  

a b

Result display with a) marked objects and b) FISH signal distribution}

 

Figure 7:  

a b c

Labelled section, a) globally segmented, b) locally improved by cutting touching nuclei, c) cleaned by deletion